Effects of nutritional enrichment on the production of acetone-butanol-ethanol (ABE) by Clostridium acetobutylicum (2024)

Choi, S. J., Lee, J., Jang, Y. S., Park, J. H., Lee, S. Y., & Kim, I. H. (2012). Effects of nutritional enrichment on the production of acetone-butanol-ethanol (ABE) by Clostridium acetobutylicum. Journal of Microbiology, 50(6), 1063-1066. https://doi.org/10.1007/s12275-012-2373-1

Choi, Sung Jun ; Lee, Joungmin ; Jang, Yu Sin et al. / Effects of nutritional enrichment on the production of acetone-butanol-ethanol (ABE) by Clostridium acetobutylicum. In: Journal of Microbiology. 2012 ; Vol. 50, No. 6. pp. 1063-1066.

@article{d70066f4f5ef41bf875cdab3de761da6,

title = "Effects of nutritional enrichment on the production of acetone-butanol-ethanol (ABE) by Clostridium acetobutylicum",

abstract = "Clostridium acetobutylicum is an industrially important organism that produces acetone-butanol-ethanol (ABE). The main objective of this study was to characterize the effects of increased cell density on the production of ABE during the phase transition from acidogenesis to solventogenesis in C. acetobutylicum. The increased ABE productivity of C. acetobutylicum was obtained by increasing the cell density using a newly designed medium (designated C. acetobutylicummedium 1; CAM1). The maximum OD600 value of C. acetobutylicum ATCC 824 strain obtained with CAM1 was 19. 7, which is 1. 8 times higher than that obtained with clostridial growth medium (CGM). The overall ABE productivity obtained in the CAM1-fermetation of the ATCC 824 strain was 0. 83 g/L/h, which is 1. 5 times higher than that (0. 55 g/L/h) obtained with CGM. However, the increased productivity obtained with CAM1 did not result in an increase in the final ABE titer, because phase transition occurred at a high titer of acids.",

keywords = "ABE, cell density, Clostridium acetobutylicum, phase transition, productivity",

author = "Choi, {Sung Jun} and Joungmin Lee and Jang, {Yu Sin} and Park, {Jin Hwan} and Lee, {Sang Yup} and Kim, {In Ho}",

year = "2012",

month = dec,

doi = "10.1007/s12275-012-2373-1",

language = "English",

volume = "50",

pages = "1063--1066",

journal = "Journal of Microbiology",

issn = "1225-8873",

number = "6",

}

Choi, SJ, Lee, J, Jang, YS, Park, JH, Lee, SY & Kim, IH 2012, 'Effects of nutritional enrichment on the production of acetone-butanol-ethanol (ABE) by Clostridium acetobutylicum', Journal of Microbiology, vol. 50, no. 6, pp. 1063-1066. https://doi.org/10.1007/s12275-012-2373-1

Effects of nutritional enrichment on the production of acetone-butanol-ethanol (ABE) by Clostridium acetobutylicum. / Choi, Sung Jun; Lee, Joungmin; Jang, Yu Sin et al.
In: Journal of Microbiology, Vol. 50, No. 6, 12.2012, p. 1063-1066.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Effects of nutritional enrichment on the production of acetone-butanol-ethanol (ABE) by Clostridium acetobutylicum

AU - Choi, Sung Jun

AU - Lee, Joungmin

AU - Jang, Yu Sin

AU - Park, Jin Hwan

AU - Lee, Sang Yup

AU - Kim, In Ho

PY - 2012/12

Y1 - 2012/12

N2 - Clostridium acetobutylicum is an industrially important organism that produces acetone-butanol-ethanol (ABE). The main objective of this study was to characterize the effects of increased cell density on the production of ABE during the phase transition from acidogenesis to solventogenesis in C. acetobutylicum. The increased ABE productivity of C. acetobutylicum was obtained by increasing the cell density using a newly designed medium (designated C. acetobutylicummedium 1; CAM1). The maximum OD600 value of C. acetobutylicum ATCC 824 strain obtained with CAM1 was 19. 7, which is 1. 8 times higher than that obtained with clostridial growth medium (CGM). The overall ABE productivity obtained in the CAM1-fermetation of the ATCC 824 strain was 0. 83 g/L/h, which is 1. 5 times higher than that (0. 55 g/L/h) obtained with CGM. However, the increased productivity obtained with CAM1 did not result in an increase in the final ABE titer, because phase transition occurred at a high titer of acids.

AB - Clostridium acetobutylicum is an industrially important organism that produces acetone-butanol-ethanol (ABE). The main objective of this study was to characterize the effects of increased cell density on the production of ABE during the phase transition from acidogenesis to solventogenesis in C. acetobutylicum. The increased ABE productivity of C. acetobutylicum was obtained by increasing the cell density using a newly designed medium (designated C. acetobutylicummedium 1; CAM1). The maximum OD600 value of C. acetobutylicum ATCC 824 strain obtained with CAM1 was 19. 7, which is 1. 8 times higher than that obtained with clostridial growth medium (CGM). The overall ABE productivity obtained in the CAM1-fermetation of the ATCC 824 strain was 0. 83 g/L/h, which is 1. 5 times higher than that (0. 55 g/L/h) obtained with CGM. However, the increased productivity obtained with CAM1 did not result in an increase in the final ABE titer, because phase transition occurred at a high titer of acids.

KW - ABE

KW - cell density

KW - Clostridium acetobutylicum

KW - phase transition

KW - productivity

UR - http://www.scopus.com/inward/record.url?scp=84871791101&partnerID=8YFLogxK

U2 - 10.1007/s12275-012-2373-1

DO - 10.1007/s12275-012-2373-1

M3 - Article

C2 - 23274997

AN - SCOPUS:84871791101

SN - 1225-8873

VL - 50

SP - 1063

EP - 1066

JO - Journal of Microbiology

JF - Journal of Microbiology

IS - 6

ER -

Choi SJ, Lee J, Jang YS, Park JH, Lee SY, Kim IH. Effects of nutritional enrichment on the production of acetone-butanol-ethanol (ABE) by Clostridium acetobutylicum. Journal of Microbiology. 2012 Dec;50(6):1063-1066. doi: 10.1007/s12275-012-2373-1